Drug Susceptibility Pattern of Extraintestinal Pathogenic E.Coli Isolated from Various Clinical Specimens

Background; Escherichia coli cause extraintestinal infections in both community and hospitalized patients. The prevalence of multidrug-resistant extraintestinal pathogenic E.coli has increased progressively over the past few years. Aim of this study is to determine the drug susceptibility pattern of Extraintestinal Pathogenic E.coli (EXPEC) isolated from various clinical isolates in the hospital. Methods; Various clinical samples processed by standard bacteriological procedures, E.coli isolated were included for the study. Antibiotic susceptibility testing was done by Kirby-Bauer disk diffusion method as per CLSI guidelines using ampicillin, ceftazidime, cefuroxime, cefotaxime, ciprofloxacin, norfloxacin, cotrimoxazole. gentamicin, Netilmycin and Nitrofurantoin for urinary isolates. Results; The isolates were highest among age group of >45 years with 45(45%), followed by 15-45 years 36(36%) and 0-15 years 19 (19%). Among the specimens Urinary isolates were 47(47.0%), exudate 30(30.0%), sputum19 (19%) and Blood 4 (4.0%). Nitrofurantoin was found to be the most sensitive among urinary isolates with susceptibility rate of 77%. Netilmycin showed highest susceptibility among isolates from all samples, blood isolates 100%, sputum 89%, urinary 60% and exudate 50% were sensitive . Gentamicin susceptibility among blood were 75%, exudate 72% and sputum was 32%. Ciprofloxacin showed highest susceptibility among Blood (50%) followed by urine (47%),. cotrimoxazole showed highest susceptibility among blood (50%). All the isolates were resistant to ampicillin and 3 generation cephalosporins. Conclusion; There was high antimicrobial resistant rate in extraintestinal E. coli infection with 3 gen cephalsporins, the results of this study highlights the importance of regular surveillance of susceptibility of E.coli.